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GM/ RP　PCR 系列相關產品

GM/ RP

PCR 系列相關產品

廠牌：GeneMark
供應商：賽恩斯生物科技股份有限公司
聯絡電話：02-28110076(代表號)
定價：與我們聯絡

快速詢價

2321

行動條碼QR Code
PCR 系列相關產品-行動條碼QR Code

產品規格
Notes

PCR Polymerases

PCR Master Mix

RT-PCR Kit

First Stranded cDNA Synthesis Kit

Quantitative RT-PCR Kit

PCR Cloning Kit

DNA Polymerases

Product Name	GenTaq DNA Polymerase	GenTaq Plus DNA Polymerase	GenHot Taq DNA Polymerase	GenFast Taq DNA Polymerase	gmPfu DNA Polymerase	LongFast Pfu DNA Polymerase	GenBest Taq DNA Polymerase
Polymerase	GenTaq	GenTaq + Pfu	Taq + Mab	Modified Taq	Modified Pfu	Modified Pfu	Modified Pfu
Proofreading	No	Yes	No	No	Yes	Yes	Yes
Product Overhang	3’-A	3’-A	3’-A	3’-A	Blunt	Blunt	Blunt
Extension	1 min/kb	1 min/kb	1 min/kb	15 sec/kb	1 min/kb	15-30 sec/kb	10~30 sec/kb
PCR product size	Up to 14 kb	Up to 14 kb	Up to 6 kb	Up to 15 kb	Up to 10 kb	Up to 10 KB	Up to 20 KB
Application	Detection	Detection Cloning Expression	Detection Multiplex PCR	Fast PCR Detection Multiplex PCR	Cloning Expression Mutagenesis	Fast PCR Cloning Expression Mutagenesis	Fast PCR Cloning Expression Mutagenesis

	產品名稱	產品編號	包裝	價格($NT)
PCR Polymerase	GenTaq DNA Polymerase	GM008	1000 U (2U/ul or 5U/ul)	1890
	GenTaq Plus DNA Polymerase	GM008P	250 U 1000 U	2048 8190
	GenHot Taq DNA Polymerase	GM008HS	200 U 1000 U	1800 7200
	GenFast Taq DNA Polymerase	GM008F	200 U 1000 U	1200 4800
	gmPfu DNA Polymerase	GPU425	250U 500U	1890 3402
	LongFast Pfu DNA Polymerase	GPU4	200U 1000U	3200 13200
	GenBest Taq DNA Polymerase	GM008B	200U 1000U	2400 9600

PCR Master Mix

	產品名稱	產品編號	包裝	價格($NT)
PCR Master Mix	PCR Master Mix (2X)	RP02	400r 2000r	2079 8316
	PCR Master Mix II	RP02 II	400r 2000r	2079 8316
	PCR Plus Master Mix II	RP02-Plus-II	100r	2394
	PCR Pfu Master Mix II	RP02-pfu-II	200r	2520
	PCR Hot-Start Master Mix II	RP02HS-II	200r	3150
PCR Dye Master Mix	PCR Dye Master Mix II (2X)	RP02D-II-B	200r 1000r	1155 4620
	PCR Dye Master Mix II (5X)	RP02D-II-A	200r 1000r	1155 4620
	2X GenFast PCR MasterMix, 1ml	RP02D-Fast	100r 500r	1800 9000
	PCR Dye Plus Master Mix	RP02D-Plus	100r	2394
	PCR Dye Pfu Master Mix	RP02D-pfu	200r	2520
	PCR Dye Hot-Start Master Mix	RP02D-HS	200r	3150

Direct PCR Kit

Cat. No.	Product	Sample	DNA Yield	Format	Operation Time
RP02-LD300	SAMtissuet Direct PCR Kit	cells, mouse tails, liver,muscle, hair shafts, skin, saliva and other animal tissues	For PCR	no DNA purification needed ; Uses pre-optimized 2X PCR mix( containing dye)	15 min
RP02-LD301	SAMbacteria Direct PCR Kit	both gram-positive and gram-negative (genomic DNA and plasmid DNA)	For PCR	no DNA purification needed ; Uses pre-optimized 2X PCR mix( containing dye)	-
RP02-LD303	SAMseed Direct PCR Kit	different kinds of seeds ; Arabidopsis, carrot, corn, cucumber, pepper, soybean, turnip, wheat and more	For PCR	no DNA purification needed ; Uses pre- 2X PCR mix( containing dye)	15 min
RP02-LD304	SAMblood Direct PCR Kit	blood, saliva, other body, fluids, buccal cells on swabs, Cultured cells, and other liquid cells	For PCR	no DNA purification ; Uses pre-optimized 2X PCR mix( containing dye)	15 min
RP02-PD8	GMplant Direct PCR Kit	plant leaves	For PCR	no DNA purification needed ; Uses pre-optimized 2X PCR mix( containing dye)	-

產品名稱	產品編號	包裝	價格($NT)
SAMtissue Direct PCR Kit	RP02-LD300	100r	5460
SAMbacteria Direct PCR Kit	RP02-LD301	100r	5460
GMplant Direct PCR Kit	RP02-PD801	100r	5460
SAMseed Direct PCR Kit	RP02-LD303	100r	5460
SAMblood Direct PCR Kit	RP02-LD304	100r	5460

Reverse transcription-PCR (RT-PCR)

> Transcriptase 反轉錄酶

> 一步法RT-PCR

> 兩步法RT-PCR

	AMV	MMLV
來源	Avian	Murine
最適作用溫度	42℃ (可有效解開二級結構)	37℃
RNase H	強	弱
合成長度	短	長

** MMLV reverse transcription 改良版 → 提高溫度耐受性

	SuperSAMscript III Reverse Transcriptase	SuperSAMscript IV Reverse Transcriptase
作用溫度	37℃~55℃ (適用於具有複雜二級結構之RNA)	42℃~65℃ (適用於具有複雜二級結構之RNA)
合成片段大小	100bp~15kb（無RNase H活性, 可增加transcripts產量）	100bp~12kb (適合長片段cDNA合成)
模板量	Total RNA: 1 ng-5 μg mRNA: 1 ng-250 ng Specific RNA: 0.01 pg-0.5 μg	Total RNA 10 ng~5 μg mRNA 1ng~500 ng Specific RNA: 0.01 pg-0.5 μg

First-Stranded cDNA Synthesis Kit

Product Name	Cat. No.	Package	Price
SuperSAMscript III First Stranded cDNA Synthesis Kit	GRS03M	50r	$3000
SuperSAMscript IV First Stranded cDNA Synthesis Kit	GRS04M	50r	$3600

One-Step RT-PCR Kit

Product Name	Cat. No.	Package	Price
One-Step RT-PCR Kit III	RP01-III	50r/250r	$3900/$16580
One-Step Plus RT-PCR Kit III	RP01-III-Plus	50r	$4500
One-Step RT-PCR Kit IV	RP01-IV	50r/250r	$4500/19130
One-Step Plus RT-PCR Kit IV	RP01-IV-Plus	50r	$4800
One-Step RT-PCR II with Dye	RP01D-II	50r	$4980

Two Step RT-PCR Kit

Product Name	Cat. No.	Package	Price
Two-Step RT-PCR kit III (SuperSAMscriptase III/GenHot)	RP012-M3	50r	$4500
Two-Step RT-PCR kit IV (SuperSAMscriptease IV/GbeHot)	RP012-M4	50r	$5000

One-Step RT-qPCR Kit

Product	Cat. No.	Enzyme
One-Step BrightGreen RT-qPCR Kit (with low ROX)	QRP01BG-LR01	SuperSAMscript III/ Hot-start Taq
One-Step BrightGreen RT-qPCR Kit (with high ROX)	QRP01BG-HR01	SuperSAMscript III/ Hot-start Taq
One-Step BrightGreen RT-qPCR Kit (without ROX)	QRP01BG-NR01	SuperSAMscript III/ Hot-start Taq

Product Name	Cat. No.	Package	Price
Green One-Step qRT-PCR Kit III	QRP01-100	100r	$4800
One-Step qRT-PCR Kit III（Probe)	QRP01P-100	100r	$5400

qPCR Master Mix

	產品名稱	產品編號	包裝	價格($NT)
qPCR Master Mix	GM SYBR qPCR Kit (2X), (High ROX)	QPSY01	1 ml	1000
	GM SYBR qPCR Kit (2X), (w/o ROX)	QPSY02	1 ml	1000
	GM SYBR qPCR Kit (2X), (Low ROX)	QPSY03	1 ml	1000
	GM Fast SYBR qPCR Kit (High ROX),	QPSY04-1	1 ml	1400
	GM Fast SYBR qPCR Kit (Low ROX),	QPSY05-1	1 ml	1400
	GM qPCR Master Mix (Probe),	QPSY01P-1	1 ml	1600
	GM Fast qPCR Master Mix (Probe)	QPSY04P-1	1 ml	1600
	BrightGreen 2X qPCR MasterMix-Low ROX	QPBG01-LR01	1 ml	1200
	BrightGreen 2X qPCR MasterMix- ROX	QPBG01-IR01	1 ml	1200
	BrightGreen 2X qPCR MasterMix-No dye	QPBG01-N01	1 ml	1200

PCR Cloning Kits & DNA Ligation Kits

TopoFast PCR Cloning Kit

Ligation只要 5 min
Blunt end or A-end 的 PCR產物都能連接
特殊工藝,不需籃白篩
Gene expression,可直接選殖到可以表現的 Topo 載體
不需要限制酵素
不需要 T4 Ligase
共價結合Topoisomerase

Cat. #	Product	Note
GBM16A	pBM16A TOPOFast PCR Cloning Kit, 20r	General TA Vector
GBM23	pBM23 TOPOFast PCR Cloning Kit, 20r	General TA Vector(including Restriction size)
GBM30	pBM30 TOPOFast PCR Cloning Kit, 20r	Gene expression Vector (prokaryotic)
GBM40	pBM40 TOPOFast PCR Cloning Kit, 20r	Gene expression Vector (Eukaryotic)

GM Seamless Cloning Kit 無縫選殖試劑盒

產品介紹：

區別於TOPO Cloning、TA Cloning 和 T4 Ligase (pGEM-T)連接等常規選殖方法，無縫選殖(Seamless Cloning)s技術可在重組酶的作用下，只需一步反應，便可將片段克隆到任何載體中的任意位置得到重組質粒。無縫選殖技術作為一種非常強大的選殖技術，具有快速、簡便、高效、多片段組裝和定向克隆等特點，用於單個 DNA 片段的克隆，多個 DNA 片段組裝克隆以及多位點突變構建等實驗目的。

產品特點：

1.簡單、快速、精確、定向克隆，連接過程只需要15分鐘。

2.只需要簡單的PCR擴增就可以製備目的片段，不需要內切酶、連接酶和磷酸化酶。

3.線性化載體的製備可以通過PCR擴增或選擇合適的內切酶酶切。

4.可以克隆長片段DNA。

DESCRIPTION	Cat.No.	REACTION
GM Seamless Cloning Kit, 20r	GMS116	20r
pUC57-simpleEVL Seamless cloning kit, 20r	GMS120	20r
2× GM SeamLess Mix, 20r	GMS117	20r

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GM/ RP　PCR 系列相關產品

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