Total RNA Miniprep Purification Kit 能抽取來自各種動植物組織、細胞與細菌的 total RNA,本試劑組以連續、快速的洗滌與離心,將細胞碎屑等雜質污染物去除,再藉由 Total RNA與 Silica membrane 的專一性結合,取得高純度的 Total RNA。
產品特性
Silica-membrane technology.
Preparation of high quality total RNA from variable sources.
Simple on-column DNase I treatment for genomic DNA removal.
High yields up to 100 μg.
No phenol / chloroform extraction, no ethanol precipitation.
實驗數據
The RNA purified from supplier Q contains large amount of genomic DNA contamination. RT-PCR of zebrafish EF-1α gene. RNA were purified by kits from GeneMark and Q supplier. In the control reaction that add Taq polymerase only with the RNA products, genomic DNA contamination was observed from Q supplier.
Non-denaturing gel electrophoresis of the total RNA purified from various samples by GeneMark and Q supplier.