BlockPRO 西方墨點法遮蓋液 Protein-free 配方不含蛋白質， 能有效降低遮蓋液與蛋白樣本的交互作用，避免物種干擾。應 用廣泛，適用於 Western blotting 與 ELISA 等免疫學實驗。

產品特點 : 

● 無蛋白質：杜絕西方墨點法遮蓋液與蛋白樣本的交互作用 ( 可用於偵測磷酸化蛋白 )

● 應用廣泛：適合各種需要西方墨點法遮蓋液的免疫學實驗 ( WB、ELISA、IHC、FACS 等實驗)

● 提高靈敏度：可減少壓片時間與抗體用量

roduct Detail :

Figure 1. BlockPRO™ Protein-Free Blocking Buffer is better than protein-based blocking buffers (skim milk, BSA and casein) for detection of target protein in Western blotting.

THP-1 cell lysates were prepared and separated by electrophoresis. The proteins were transferred to PVDF and blocked for 1 hour at room temperature with the indicated blocking buffer, probed with mouse anti-pAMPK followed by anti-mouse HRP and detected by chemiluminescence. All results were exposed to X-ray film for 30 seconds.

Figure 2. BlockPRO™ Protein-Free is suitable for various protein antigen detection, such as high molecular weight protein, tACC; phosphoprotein, pAMPK; abundant protein, GAPDH; low molecular weight protein, histone H3.

THP-1 cell lysates were prepared and separated by electrophoresis. The proteins were transferred to PVDF and blocked for 1 hour at room temperature with BlockPRO™ Protein-Free Blocking Buffer. Antibodies designed to probe the indicated proteins were used. All the signals were detected by chemiluminescence and were exposed to X-ray film.

Figure  3. BlockPRO™ Protein-Free Blocking Buffer can be used in both PVDF and nitrocellulose platform. 

Hela cell lysates were prepared and separated by SDS-PAGE. The proteins were transferred to PVDF or nitrocellulose membranes. The membranes were blocked for overnight at 4 °C with BlockPRO™ Protein-Free Blocking Buffer or 5% skim milk, probed with mouse anti-histone H3 followed by anti-mouse HRP and detected by chemiluminescence. All results were exposed to X-ray film for 30 seconds.

訂購資訊 :