TOPfast PCR Cloning Kit is suitable for cloning Taq polymerase amplified PCR products (with A-tail overhangs) at room temperature in 5 min. The vector provided in this kit contains topoisomerase covalently coupled to its ends, which allows efficient ligation of DNA fragments with A-tail overhangs to the vector without using a regular ligase.
產品資訊
《快速簡單》
《好用、高效率》
Figure 1. Colony PCR results from colonies carrying different sized inserts.
PCR products of 300 bp (50 ng/µl)、1 kb (50 ng/µl) 、2 kb (100 ng/µl) and 3 kb (150 ng/µl) were ligated to TOPfast PCR Cloning Vectors, and colony PCR was performed using M13 primers.
For successful clones, the PCR result should be ~202 bp plus the insert size.
M : Gen-KB DNA Ladder
TOPfast Vector Information
產品內容物
1 vial of 20 μl | TOPfast Vector (10 ng/μl) |
1 vial of 20 μl | 6X TOPfastTM Buffer |
1 vial of 5 μl | Control insert DNA (1 kb) |
21 vials of 100 μl | DH5α Competent Cell |
1 vials 5 μl | Control Plasmid |
1 vial of 400 μl | M13 Control primer mix |
1 vial of 7.5 ml | SOC medium |
1 vial of 1 ml | Sterile water |
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