BICINE 生物緩衝劑
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Catalog No.Bio-OT005 | |
CAS No. | 150-25-4 ( CAS No. is listed on the TSCA inventory ) |
EC No. | 205-755-1 |
Synonyms | N, N-Bis ( 2-Hydroxyethyl ) glycine； N, N-Di ( 2-Hydroxyethyl ) glycine； N, N-Dihydroxyethyl Glycine； [ Bis-( 2-Hydroxy-ethyl )-amino ]-acetic acid； N, N-Dihydroxyethylglycine； Diethylolglycine； DHEG |
Chinese Name | N, N-二羥乙基甘氨酸 |
Molecular Structure | |
Molecular Formula | C6H13NO4 |
Molecular Weight | 163.2 |
Meleing point | 194℃ |
Water solubility | Solubility ( 0.1M in H2O ) clear and complete |
pKa ( 25℃ ) | 8.3 ± 0.2 |
Appearance | White powder |
Purity Assay | ≥ 99.0% ( Titration ) |
Moisture | ≤ 1.0% |
UV A260 / A280nm | UV ( 0.1 M in H2O ) A260nm ≤ 0.05 A280 ≤ 0.05 |
Storages | Room Temperature |
Description | 1. Bicine is a zwitterionic biological buffer. 2. Bicine is a versatile buffer. Its applicationsinclude enzyme reaction buffers and electrophoresis buffers. Bicine interferes with the BCA and Lowry protein assay. Ferricyanide slowly oxidized bicine. In addition, its strong binding of copper ions may be disturbing. The working concentration lasts from 3 to 100mM. 3. It is a suitable buffer for low temperature biochemical work. Used in the preparation of stable substrate solution for serum guanase determination. Also as a spacer in plasma protein fractionation with Sephadex by isotachophoresis. 4. Bicine is used in a variety of molecular biology applications such as protein resolution, protein crystallation and SDS-PAGE. |
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Useful pH Ranges of Selected Biological Buffers (25℃, 0.1M)
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Our biological buffers are used widely in life science and pharmaceuticals including cell biology, molecular biology and industrial biopharmaceutical applications. The products are non toxic to cell cultures, and have pKa values at or near physiological pH. All of our buffer are at least the ultra pure grade, which have high assay, solubility and low heavy metals, and free of DNase, RNase, and Protease.
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